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Quick-gDNA™ MidiPrep---快速-基因组DNA中量提取试剂盒
About Quick-gDNA™ MidiPrep
The Quick-gDNA™ MidiPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, cells from culture, and many biological liquid samples.
For processing, simply add the specially formulated Genomic Lysis Buffer to a sample, vortex, and transfer the mixture to the supplied Zymo-Spin™ Column w/ Zymo-Midi Filter™. There is no need for organic denaturants or Proteinase K digestion because of the unique lysis buffer system. The product features Zymo-Spin™ technology to yield high-quality, purified DNA in just 20 minutes (see below). PCR inhibitors are effectively removed during the purification process. DNA purified using theQuick-gDNA™ MidiPrep is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
| DNA Recovery |
Up to 125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. |
| Equipment |
Centrifuge or vacuum source and manifold, microcentrifuge, vortex. |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
Up to 3 ml (see protocols) whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed. |
| DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| Workflow Overview |
Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
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For the purification of high quality DNA from up to 3 ml whole blood, plasma, serum, body fluids (e.g., semen) and buffy coat, lymphocytes, swabs and cultured cells in less than 20 minutes using innovative Zymo-Spin™ column technology. Up to 125 µg/prep.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants..
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| Product |
Size |
Catalog # |
How to Buy |
| Quick-gDNA™ MidiPrep |
25 Preps |
D3100 |
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