The DNA Clean & Concentrator™-5 product provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
| Step 1 |
In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below). Mix briefly by vortexing.
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Application
|
DNA Binding Buffer : Sample
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Example
|
|
Plasmid, genomic DNA (>2 kb)
|
2 : 1
|
200 µl : 100 µl
|
|
PCR product, DNA fragment
|
5 : 1
|
500 µl : 100 µl
|
|
ssDNA1 (e.g. cDNA, M13 phage)
|
7 : 1
|
700 µl : 100 µl
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For efficient recovery of genomic or large DNA (< 20 kb to < 200 kb), use the Genomic DNA Clean & Concentrator™ (Cat. Nos. D4010, D4011).
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| Step 2 |
Transfer mixture to a provided Zymo-Spin™ Column2 in a Collection Tube.
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| Step 3 |
Centrifuge for 30 seconds. Discard the flow-through.
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| Step 4 |
Add 200 µl DNA Wash Buffer to the column. Centrifuge for 30 seconds. Repeat the wash step.
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| Step 5 |
Add ≥ 6 µl DNA Elution Buffer3 or water4 directly
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Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
| DNA Clean & Concentrator™-5 - Uncapped Columns |
50 Preps |
D4003 |
 |
| DNA Clean & Concentrator™-5 - Uncapped Columns |
200 Preps |
D4004 |
|
| DNA Clean & Concentrator™-5 - Capped Columns |
50 Preps |
D4013 |
|
| DNA Clean & Concentrator™-5 - Capped Columns |
200 Preps |
D4014 |
