The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product featuresFast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment |
Microcentrifuge |
| DNA Size Limits |
50 bp to 23 kb |
| Sample Sources |
DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| DNA Purity |
High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
 |
The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.
|
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
| DNA Clean & Concentrator™- 25 - Uncapped Columns |
50 Preps |
D4005 |
 |
| DNA Clean & Concentrator™- 25 - Uncapped Columns |
200 Preps |
D4006 |
|
| DNA Clean & Concentrator™- 25 - Capped Columns |
50 Preps |
D4033 |
|
| DNA Clean & Concentrator™- 25 - Capped Columns |
200 Preps |
D4034 |
|
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