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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR Plasmid Miniprep™ - Classic---质粒小量提取试剂盒 Classic
About ZR Plasmid Miniprep™ - Classic
The ZR Plasmid Miniprep™-Classic is designed for efficient isolation of plasmid DNA from E. Coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast-Spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. The innovative Zymo-Spin™ llN columns yield endotoxin-free plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
| Format |
Spin Column |
| DNA Recovery |
≥ 30 µl |
| Processing Time |
15 min |
| DNA Purity |
Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) ≥ 1.8. |
| Plasmid DNA Size |
Up to 25 kb. |
| Plasmid DNA Yield |
Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
| Procedure |
Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold. |
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Plasmid products. Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep™-Classic, performed in triplicate. M: ZR 1 kb DNA marker (Zymo Research)
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Sequence-quality DNA preparations. DNA sequencing chromatogram of plasmid DNA prepared using the ZR Plasmid Miniprep™-Classic.
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For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc..
Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps
Unique column design: zero buffer retention and low (30 µl) elution volume..
| ZR Plasmid Miniprep™- Classic |
100 Preps. |
D4015 |
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| ZR Plasmid Miniprep™- Classic |
400 Preps. |
D4016 |
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| ZR Plasmid Miniprep™- Classic |
800 Preps. |
D4054 |
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