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Epigenetics |
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| Epigenetics>【Zymo】 |
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5-mC DNA ELISA Kit
About 5-mC DNA ELISA Kit
The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research. To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation.
The 5-mC DNA ELISA Kit is a convenient and powerful tool that allows the researcher to accurately quantitate 5-mC in anyDNA sample in less than 3 hours. The kit features a unique Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.
| Equipment |
Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps. |
| DNA Size Limits |
This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng. |
| Sample Sources |
Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar. |
| Detection |
≥ 0.5% 5-methylcytosine (5-mC) per 100 ng single-stranded DNA. |
For high-throughput, detection of global 5-methylcytosine (5-mC) in DNA.
The streamlined workflow can be completed in less than 3 hours.
| 5-mC DNA ELISA Kit |
1 x 96 |
D5325 |
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| 5-mC DNA ELISA Kit |
2 x 96 |
D5326 |
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表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
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