The ZR Soil Microbe DNA MiniPrep™ is designed for the simple and rapid isolation of humic-free, PCR-quality DNA from microbes in soil. This product can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a variety of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil microbes are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. The procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to 25 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time |
15 min |
| Equipment |
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
DNA is isolated from bacteria, fungi, protozoa, and algae in up to 0.25 g of soil or from fungal/bacterial cells directly. |
| DNA Purity |
High-quality, humic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280>1.8 |
 |
Metagenomic DNA isolated from 5 soil samples. M: 1 kb marker (NEB); 1-5: soil samples (sand, sandy clay loam, hydrophobic sandy loam, course sandy loam, fine gravel).
|
Simple, efficient isolation of humic-free DNA from microbes in soil, sludge, sediment, sand, and water in minutes including tough-to-lyse bacteria, fungi, algae, and protozoa.
Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
| ZR Soil Microbe DNA MiniPrep™ |
50 Preps. |
D6001 |
 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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