The ZR Fungal/Bacterial DNA MiniPrep™ is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to 25 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time |
10 min |
| Equipment |
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
50-100 mg (wet weight) fungi or bacteria or up to 200 mg tissue. This equates to approximately 109bacterial cells, 108 yeast cells and 107 mammalian cells. Spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| DNA Purity |
High quality DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
 |
Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. Coliusing the ZR Fungal/Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).
|
Simple, efficient isolation of DNA (up to 25 µg/prep) from all types of tough-to-lyse fungi (e.g., yeast) and bacteria in as little as 15 minutes.
State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
Can be used with any bead mill, disrupter, or vortex that can accommodate standard 2 ml tubes.
Omits the use of organic denaturants as well as proteinases.
| ZR Fungal/Bacterial DNA MiniPrep™ |
50 Preps. |
D6005 |
 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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