ZR Soil Microbe DNA MidiPrep™---土壤微生物DNA中量提取试剂盒
About ZR Soil Microbe DNA MidiPrep™
The ZR Soil Microbe DNA MidiPrep™ is designed for the simple and rapid isolation of humic-free, PCR-quality DNA from microbes in soil. This product can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a variety of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil microbes are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. The procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to ~125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer per sample. |
| Processing Time |
20 min |
| Equipment |
Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
| DNA Size Limits |
Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered. |
| Sample Sources |
DNA is isolated from bacteria, fungi, protozoa, and algae in up to 5 g of soil. However, 2.5 g is recommended for most applications. The amount of sample will vary depending on the composition of the sample: process more material for wet muddy samples and less for dry sandy samples. DNA can also be isolated directly from isolated (pelleted) fungus and bacteria (see protocol). |
| DNA Purity |
High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/280 >1.8, A260/230 >2.0 |
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Metagenomic DNA isolated from 5 soil samples. M: 1 kb marker (NEB); 1-5: soil samples (sand, sandy clay loam, hydrophobic sandy loam, course sandy loam, fine gravel).
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Simple, efficient isolation of humic-free DNA from microbes in soil, sludge, sediment, sand, and water in minutes including tough-to-lyse bacteria, fungi, algae, and protozoa.
Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
| Product |
Size |
Catalog # |
How to Buy |
| ZR Soil Microbe DNA MidiPrep™ |
25 Preps. |
D6101 |
 |
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