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Epigenetics |
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Epigenetics>【Zymo】 |
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ZR Plant/Seed DNA MidiPrep™---植物/种子DNA中量提取试剂盒
About ZR Plant/Seed DNA MidiPrep™
This product has been discontinued. Please see the ZR Plant/Seed DNA Miniprep for DNA isolation from plant samples.
Click here to view
Format |
Spin Column |
DNA Recovery |
Up to ~125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer per sample. |
Processing Time |
25 Min |
Equipment |
Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
DNA Size Limits |
Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered. |
Sample Sources |
Up to 375 mg that include leaves, stems, buds, flowers, fruit, seeds, etc. |
DNA Purity |
High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/280 >1.8, A260/230 > 2.0 |
DNA Yield |
Typically 20-80 ng DNA/mg plant material is recovered. |
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Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA MiniPrep™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), juniper (2), corn kernel (3, 4), sunflower seed (5, 6).
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Simple methods for the isolation of DNA from tough-to-lyse plant and seed samples in minutes.
Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
Fast-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Product |
Size |
Catalog # |
How to Buy |
ZR Plant/Seed DNA MidiPrep™ |
25 Preps. |
D6120 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
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