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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR-Duet™ DNA/RNA MiniPrep (Plus)（D7001） |
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ZR-Duet™ DNA/RNA MiniPrep (Plus)
About ZR-Duet™ DNA/RNA MiniPrep (Plus)
| MiniPrep Plus |
MiniPrep |
Cells
Any tissue
(soft, lipid, fibrous, FFPE)
Whole blood |
Cells
Soft tissue |
| Included |
DNase I
gDNA removal column
Proteinase K
DNA/RNA Shield (for sample storage) |
gDNA removal column |
The ZR-Duet™ DNA/RNA kits provide a quick method for the isolation of high quality genomic DNA and total RNA from cells, tissue, blood, and biological fluids. The kits isolate both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs and microRNAs ≥17nt are also recovered – no extra steps required!
| Sample Sources |
Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and samples in DNA/RNA Shield™. |
| Storage |
DNA and RNA is eluted with DNase/RNase-free water and can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Sample Size |
Up to 107 cells in suspension or up to 50 mg of tissue. |
| Purity |
High quality genomic DNA and total RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered. |
| Size Limit |
Capable of recovering genomic DNA ≥40 kb. Total RNA ≥17 nt can be recovered. |
| Equipment Needed |
Microcentrifuge, vortex, and 55°C heat block, water bath or incubator. |
| Recovery |
The DNA/RNA binding capacity of the included columns is 100 µg. |
| Sample Preservation |
DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
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Genomic DNA (lane 1, 2) and total RNA (lane 3, 4) isolated from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep.
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PCR amplification of β-actin transcript (353 bp fragment shown) following DNA and RNA isolation from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep: PCR positive control (DNA template; lane 1, 2, 3), PCR negative control (RNA template; lane 4, 5, 6), RT-PCR (lane 7, 8, 9).
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Efficient isolation and separation of DNA and RNA from any cells, tissue, blood, and biological fluids.
High quality DNA and DNA-free RNA is ready for use in any downstream application. DNase I included (Plus kit only)
| NEW ZR-Duet ™ DNA/RNA MiniPrep Plus |
50 Preps |
D7003 |
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| ZR-Duet™ DNA/RNA MiniPrep |
50 Preps |
D7001 |
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