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Epigenetics |
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Epigenetics>【Zymo】 |
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ZR & ZR-96 Viral DNA/RNA Kits™(D7020) |
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ZR & ZR-96 Viral DNA/RNA Kits™
About ZR & ZR-96 Viral DNA/RNA Kits™
The ZR Viral DNA/RNA Kit™ provide for rapid isolation of high-quality viral nucleic acids from a wide range of biological sources. The kits can be used to successfully isolate viral DNA and RNA from cell-free body fluids (plasma, serum, etc.) as well as cellular suspensions at concentrations ≤ 1 x 105 cells/ml. The procedure employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA binding onto the column/plate matrix. The nucleic acids are washed then eluted with DNase/RNase-free Water. The eluted DNA and RNA are suitable for use in various subsequent procedures including RT/PCR.
Format |
Spin Column / 96-well spin plate |
Equipment |
Microcentrifuge (column) / centrifuge with 96-well plate carrier (plate). |
Sample Sources |
Plasma, serum, culture supernatants, animal cells and tissue. |
Elution Volume |
≥35 μl (column) / ≥10 μl (plate) |
Storage |
Eluted DNA/RNA can be stored at ≤ -70°C |
Sample Size |
≤200 µl |
Size Limit |
DNA/RNA fragments >50 bases. |
The ZR Viral Kits™ from Zymo Research ensures high sensitivity viral detection compared to that of Supplier Q. Viral RNA was isolated from plasma samples using the ZR Viral RNA Kit™. Data are the mean (+/- SD) of triplicate RT-qPCR measurements.
Quick co-purification of viral DNA/RNA from a wide range of sources (plasma, serum, etc.)
Zymo-Spin column & 96-well plate technology allows ultra-clean DNA and RNA to be eluted into minimal volumes.
Omits the use of organic denaturants and proteases
ZR Viral DNA/RNA Kit™ |
25 Preps |
D7020 |
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ZR Viral DNA/RNA Kit™ |
100 Preps |
D7021 |
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ZR-96 Viral DNA/RNA Kit™ |
2x96 Preps |
D7022 |
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ZR-96 Viral DNA/RNA Kit™ |
4x96 Preps |
D7023 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
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