Zymolyase - Yeast Lytic Enzyme---酵母裂解酶
About Zymolyase - Yeast Lytic Enzyme
Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus, lyophilized, and packaged with a resuspension buffer. The buffer has been optimized to confer maximal levels of enzymatic activity. The main activities of the enzyme are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase, which hydrolyze glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. Optimal Zymolyase activity is at 30° - 37°C; lytic activity ceases at higher temperatures. R-Zymolyase includes 0.5 U/µl RNase A when reconstituted. Susceptible fungal genera: Asbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Saccharomyces, Saccharomycodes, Saccharomycopsis, Schizosaccahromyces, Torulopsis.
| Format |
Lyophilized enzyme provided w/ storage buffer |
| Concentration |
5 U/µl |
| Source |
Arthrobactor luteus |
| Storage |
Zymolyase is stable for over 1 year at -20°C or many years below -70°C. |
| Unit Definition |
One lytic unit is defined as catalyzing a 10% decrease in optical density at 800 nm (OD800) at 30°C in 30 minutes. |
| Optimum pH and Temperature |
pH 7.5, 35°C (lysis of viable yeast), pH 6.5, 45°C (hydrolysis of yeast glucan). |
| Activity |
ß-1,3-glucan laminaripentaohydrolase and ß-1,3-glucanase (trace amount of protease, ca. 1.5 units per 10 µl). DNase and RNase: none detectable. |
| Protein Content |
~10-15 mg/ml |
| Deactivation |
Lytic activity is lost in 5 minutes at 60°C. |
| Assay Condition |
50 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol in 1 ml yeast cell suspension of 0.8 - 1.0 OD800. |
|
Zymolyase can be used for enzymatic digestion of yeast glycan coats and for spheroplast formation. The arrow indicates the nucleus and intracellular components of a spheroplast through a partially digested plasma membrane.*
*Source: A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM). Elena Kiseleva, Terry D Allen, Sandra A Rutherford, Steve Murray, Ksenia Morozova, Fiona Gardiner, Martin W Goldberg & Sheona P Drummond. Nature Protocols 2, 1943 - 1953 (2007) Published online: 9 August 2007 doi:10.1038/nprot.2007.251
|
Zymolyase (100T equivalent) prepared from Arthrobacter luteus (essential enzyme activities: β-1,3-glucan laminaripentao hydrolase and β-1,3-glucanase).
Provided lyophilized together with a buffer for reconstitution.
Also available combined with RNase A (R-Zymolyase).
| Zymolyase (includes 500µl storage buffer) |
1,000 Units |
E1004 |
 |
| Zymolyase (includes 500µl storage buffer) |
2,000 Units |
E1005 |
|
| R-Zymolyase (includes 500µl storage buffer) |
1,000 Units |
E1006 |
|
精品推荐：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
| RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过 |
