| Storage |
Store at -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents. |
| Unit Definition |
One unit (U) enzyme of ZymoTaq™ DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C. |
| Enzyme Concentration |
Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq™ DNA polymerase. |
Real-Time Amplification Plot. This figure shows robust real-time amplification of 10 ng of bisulfite-converted human DNA using the ZymoTaq™ qPCR PreMix and primers designed to amplify a region of the MGMT promoter.
Reliable Standards for the Quantification of Human DNA: Human DNA Standards performed in duplicates 10-fold serial dilutions ranging from 20 ng to 20 fg.
