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Epigenetics |
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| Epigenetics>【Zymo】 |
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His-Spin Protein Miniprep™（P2001） |
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His-Spin Protein Miniprep™
About His-Spin Protein Miniprep™
The His-Spin Protein Miniprep™ provides researchers with a method for fast His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification, and uniqueFast-Spin column technology. Up to 1 mg of His-tagged protein can be purified in as little as 5 minutes and can be eluted into as little as 100 µl of the provided His-Elution Buffer. The purified protein can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE, as well as other protein based applications. The His-Spin Protein Miniprep™ has been optimized to yield maximal protein purity indices: a single protein band is often visualized following Coomassie Blue® staining of proteins in SDS-PAGE gel (see figure below). The straightforward spin-wash-elute protocol dramatically simplifies protein purification and results are obtained in minutes, not hours!
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Purification of 6X His-fusion proteins. E. coli cell extracts, containing indicated proteins (i.e., 112, 32, 18 kDa) expressed as a N-terminal 6X His-fusion, as well as the proteins purified using His-Spin Protein Miniprep™ were analyzed by SDS-PAGE in a 15% (w/v) polyacrylamide gel, and stained with Coomassie Blue®. The recombinant proteins were purposely expressed to a low level to demonstrate the efficiency of the His-Spin Protein Miniprep™.
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Fast (5 minute) method for the purification of His-tagged proteins from cell free extracts.
Screen bacterial colonies directly on the basis of protein expression vs. plasmid DNA.
No special instrumentation is required other than a benchtop microcentrifuge.
| His-Spin Protein Miniprep™ |
10 Preps. |
P2001 |
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| His-Spin Protein Miniprep™ |
50 Preps. |
P2002 |
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关于5-hmC
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