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Epigenetics |
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| Epigenetics>【Zymo】 |
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RNA Clean & Concentrator™-5
About RNA Clean & Concentrator™-5
RNA Clean & Concentrator™-5 provides a simple and reliable method for the rapid preparation of up to 10 µg of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).
The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
The entire procedure typically takes about 5 minutes.
| Sample Sources |
DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol®/chloroform or similar extraction. |
| RNA Purity |
High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for reverse transcription, microarray, sequencing etc. |
| RNA Recovery |
Up to 10 μg RNA can be eluted into as little as ≥6 μl RNase-free water allowing for a highly concentrated sample. |
| RNA Storage |
RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| Equipment Needed |
Microcentrifuge |
| RNA Size Limits |
From 17 nt to ~23 kb. |
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Concentration of diluted RNA samples.
RNA was eluted with 20 μl RNase-free water (n = 3, total input = 1 μg RNA). |
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Purification of small and large RNAs into separate fractions.
RNA Clean & Concentrator™ allows for purification of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). |
Quick, 5 minute recovery of ultra pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following Trizol® extraction) and other sources.
High-quality RNA eluted in ≥6 μl is ready for reverse transcription, microarray, sequencing, etc.
| RNA Clean & Concentrator™-5 |
50 Preps |
R1015 |
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| RNA Clean & Concentrator™-5 (supplied with DNase I) |
50 Preps |
R1013 |
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| RNA Clean & Concentrator™-5 |
200 Preps |
R1016 |
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| RNA Clean & Concentrator™-5 (supplied with DNase I) |
200 Preps |
R1014 |
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