The ZR Viral RNA Kit™provides for rapid isolation of high-quality viral RNA from a wide range of biological sources. It can be used to successfully isolate viral RNA from cell-free body fluids as well as cellular suspensions at concentrations ≤1x105cells/ml. The kit has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus and parvovirus (a ssDNA virus).
The ZR Viral RNA Kit™employs a single buffer system that facilitates viral particle lysis and allows for RNA adsorption onto the matrix of the Zymo-Spin™Column. The RNA is washed then eluted with DNase/RNase-Free Water. The eluted RNA is suitable for use in various subsequent procedures including RT-PCR.
The entire RNA isolation procedure typically takes about 5 minutes.
| Equipment |
Microcentrifuge |
| Sample Sources |
Plasma, serum, culture supernatants, animal cells and tissue. |
| RNA Purity |
High-quality RNA suitable for reverse transcription, etc. |
| RNA Recovery |
Up to 10 μg RNA can be eluted into as little as 6 μl RNase-free water allowing for a highly concentrated sample. |
| Sample Size |
≤200 µl |
|
RT-PCR amplification of enterovirus cDNA. Human serum was spiked with different amounts of infectious enterovirus, then viral RNA was extracted using the ZR Viral RNA Kit™. The eluted RNA was used for one-tube RT-PCR amplification of a 230 bp amplicon. M is a 100 bp DNA Marker (Zymo Research)
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| |
| |
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Viral RNA was isolated from liquid samples using the ZR Viral RNA Kit™. Isolated viral RNA was reverse transcribed/amplified using a coupled RT-real-time PCR system (Zymo Research). Ct values for measles and influenza type A (FluA) were 23.05 (diamonds), 24.56 (triangles), respectively.
|
Quick, 5-minute recovery of viral RNA from plasma, serum and other samples.
Omits the use of organic denaturants and proteases.
High-quality RNA is eluted in ≥6 μl and ready for RT-PCR and other downstream analyses.
| ZR Viral RNA Kit™ |
50 Preps. |
R1034 |
 |
| ZR Viral RNA Kit™ |
200 Preps. |
R1035 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
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