Quick-RNA™ MiniPrep (Plus)---快速RNA小量提取试剂盒
About Quick-RNA™ MiniPrep (Plus)
| MiniPrep Plus |
MiniPrep |
Cells
Any tissue
(soft, lipid, fibrous, FFPE)
Whole blood |
Cells
Soft tissue |
| Included |
DNase I
gDNA removal column
Proteinase K
DNA/RNA Shield (for sample storage) |
DNAse I
gDNA removal column |
The Quick-RNA™ MiniPrep kits are innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system withZymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes.
The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
| Sample Sources |
Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and enzymatic reactions (e.g., DNase I), samples in DNA/RNA Shield™, TRIzol® or RNAlater™. |
| Storage |
Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification. |
| RNA Purity |
High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery |
Up to 100 µg RNA can be eluted into ≥30 µl RNase-free water allowing for a highly concentrated sample. |
| Sample Size |
Up to 107 cells or 50 mg tissue. |
| RNA Storage |
RNA is eluted with RNase-free water and can be stored frozen. |
| Equipment Needed |
Microcentrifuge |
| Sample Preservation |
DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
|
Universal
The Quick-RNA™ MiniPrep Plus kit is universal and accommodates any sample input including cells, tissue, blood, etc.
|
 |
|
High Quality RNA
High quality RNA is consistently isolated with theQuick-RNA™ MiniPrep Plus kit (left), not with kits as recommended by supplier Q (right). Quality assessed by Agilent 2200 TapeStation; red = low quality
|
 |
DNA-free RNA
RNA isolated with the Quick-RNA™ MiniPrep Plus kit is DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.
|
 |
Purify high-quality total RNA (including small/micro RNAs) from any cells, tissues, blood and biological fluids.
DNA-freeRNA is ready for use in any downstream application. DNase I included.
| NEW Quick-RNA™ MiniPrep Plus |
50 Preps |
R1057 |
 |
| NEW Quick-RNA™ MiniPrep Plus |
200 Preps |
R1058 |
|
| Quick-RNA™ MiniPrep |
50 Preps |
R1054 |
|
| Quick-RNA™ MiniPrep |
200 Preps |
R1055 |
|
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