DNA/RNA Shield™
About DNA/RNA Shield™
DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures. There is no need for refrigeration or specialized equipment. DNA/RNA Shield™ effectively lyses cells and inactivates nucleases and infectious agents (virus), and it is compatible with various collection and storage devices (vacutainers, swabs, nasal, buccal, fecal etc.).
| DNA/RNA Shield™ |
Recommended |
| R1100-50, R1100-250 |
Cells, Tissue, Swabs, Stool etc. |
| |
Samples should not exceed 10% (v/v). |
R1200-25,R1200-125
2X concentrate |
Blood, cells in suspension and biological liquids |
| |
Mix an equal volume reagent and sample. |
RNA Stabilization
 |
Nucleic Acid Purification
 |
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Cellular RNA is effectively stabilized in DNA/RNA Shield™ at ambient temperature. RNA was purified from cells (HCT 116) stored in DNA/RNA Shield™ at the designated times (days).
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High quality DNA and RNA is efficiently purified from blood stored in DNA/RNA Shield™ using the Quick-gDNA™ and Quick-RNA™ Kits from Zymo Research, respectively. High molecular weight DNA was intact with no apparent degradation. Also, RNA was high quality, DNA-free, and included small RNAs when purified from DNA/RNA Shield™. This was not the case using another reagent/kit from Supplier B.
|
Virus Inactivation
|
 |
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Influenza virus is effectively inactivated by DNA/RNA Shield™. Influenza A virus was treated with four volumes of DNA/RNA Shield™ or mock treated for 5 minutes. Titer was subsequently determined by plaque assay. (D. Poole and A. Mehle, University of Wisconsin-Madison)
Ebola virus is effectively inactivated by DNA/RNA Shield™. Ebola virus (Kikwit) was treated with two volumes of DNA/RNA Shield™ or PBS for 5 minutes at ambient temperature. Titer was subsequently determined by plaque assay. (L. Avena and A. Griffiths, Texas Biomedical Research Institute)
Herpes Simplex Virus is effectively inactivated by DNA/RNA Shield™. HSV-1 (dark gray) & HSV-2 (light gray) inactivation following a 5 minute incubation in the DNA/RNA Shield™. (H. Oh, F. Diaz and D. Knipe; Harvard Medical School)
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Preserves genetic integrity and expression profiles of samples (cells, tissues, blood, plasma, serum, saliva, urine, feces etc.) at ambient temperatures.
DNA and RNA can be isolated directly without precipitation or reagent removal (compatible with most DNA and RNA purification kits).
Inactivates infectious agents (virus).
| DNA/RNA Shield™ |
50 ml |
R1100-50 |
 |
| DNA/RNA Shield™ |
250 ml |
R1100-250 |
|
| DNA/RNA Shield™ (2X concentrate) |
25 ml |
R1200-25 |
|
| DNA/RNA Shield™ (2X concentrate) |
125 ml |
R1200-125 |
|
| DNA/RNA Shield™ - Lysis Tube (Microbe) |
50 Pack |
R1100-1-B15 |
|
| DNA/RNA Shield™ - Lysis Tube (Tissue) |
50 Pack |
R1100-1-B2 |
|
| DNA/RNA Shield™ - Collection Tube |
50 Pack |
R1100-1-T |
|
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