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Epigenetics |
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| Epigenetics>【Zymo】 |
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真菌/细菌基因组RNA小量提取试剂盒（R2014） |
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ZR Fungal/Bacterial RNA MiniPrep™---真菌/细菌基因组RNA小量提取试剂盒
About ZR Fungal/Bacterial RNA MiniPrep™
The ZR Fungal/Bacterial RNA MiniPrep™ provides for rapid isolation of RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast or fungal cells. The ZR Fungal/Bacterial RNA MiniPrep™ employs ultra-high density ZR BashingBeads™ for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples.
The Zymo-Spin™ IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IIC Columnefficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
RNA can be eluted into volumes as little as 25 µl in less than 10 minutes.
| Format |
Bead beating, spin column. |
| Equipment |
Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional). |
| Sample Sources |
50-100 mg (wet weight) fungi or bacteria. This equates to approximately 109 bacterial cells and 108yeast cells. |
| RNA Purity |
High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. In general, traces of DNA may be present in the eluted RNA fraction. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion. |
| RNA Recovery |
RNA can be eluted into small volumes, ≥25 µl, allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~50 µg. |
| Compatibility |
Compatible with samples stored in RNAlater™. |
| RNA Storage |
RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pGEM®) using the ZR Fungal/Bacterial RNA MicroPrep™ or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.
* = genomic (< 10 kb) and plasmid (< 3 kb) DNA contamination;
DNase I = samples treated with DNase I.
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Quick, 10 minute isolation of total RNA (~50 µg) from Gram-negative/positive bacteria, yeast and fungi using ultra-high density BashingBeads™ and Zymo-Spin™ column technologies.
High-quality RNA eluted in ≥6 µl is ready for reverse transcription, microarray, sequencing, etc.
| Product |
Size |
Catalog # |
How to Buy |
| ZR Fungal/Bacterial RNA MiniPrep™ |
50 Preps |
R2014 |
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