Direct-zol™ RNA Kits
About Direct-zol™ RNA Kits
Special Offer
20% off the NEW Direct-zol™ MiniPrep Plus!
Use the Promo Code DZ20OFF at checkout
*Special offer valid from June 7, 2016 through December 31, 2016 to direct US customers only. Offer not valid towards previous purchases and cannot be combined with other promotions. Other exclusions may apply.
| |
MiniPrep Plus |
MiniPrep |
MicroPrep |
| RNA Recovery |
100 µg |
50 µg |
10 µg |
| Minimum Elution |
50 µl |
25 µl |
6 µl |
| (Animal) Cells |
≤ 107 |
≤ 5 x 106 |
≤ 106 |
| Tissue |
≤ 50 mg |
≤ 25 mg |
≤ 5 mg |
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNAdirectly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below).
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
The entire procedure typically takes only 7 minutes.
*U.S. Patent No. 9,051,563 and other pending patents.
| Equipment |
Microcentrifuge |
| Sample Sources |
Any sample stored and preserved in TRI Reagent®, TRIzol® or similar. (animal cells, tissue, bacteria, yeast, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| RNA Purity |
A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA can be accomplished using an in-column DNase I digestion |
| Compatibility |
TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
| RNA Storage |
RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| RNA Size Limits |
RNAs ≥17 nucleotides. |
| Sample Inactivation |
TRI Reagent® (provided with R2051, R2053, R2061, R2063, R2071, R2073 only) inhibits RNase activity and inactivates viruses and other infectious agents. |
 |
 |
| High quality broad range RNA is purified with the Direct-zol™ RNA MiniPrep. (A) DNA-free RNA purified from human epithelial cells using the Direct-zol™ RNA MiniPrep compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). (B) Small RNAs are effectively recovered with the Direct-zol™ procedure while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown) |
The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to an unbiased method (mirVana™, Ambion). Micro-RNA analysis was performed using miRNA-Seq (MiSeq®, Illumina) and a direct hybridization assay (nCounter®, Nanostring). |
Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.
| NEW Direct-zol™ RNA MiniPrep Plus |
50 Preps |
R2070 |
 |
| NEW Direct-zol™ RNA MiniPrep Plus |
200 Preps |
R2072 |
|
| NEW Direct-zol™ RNA MiniPrep Plus w/ TRI Reagent® |
50 Preps |
R2071 |
|
| NEW Direct-zol™ RNA MiniPrep Plus w/ TRI Reagent® |
200 Preps |
R2073 |
|
| Direct-zol™ RNA MiniPrep |
50 Preps |
R2050 |
|
| Direct-zol™ RNA MiniPrep |
200 Preps |
R2052 |
|
| Direct-zol™ RNA MiniPrep w/ TRI-Reagent® |
50 Preps |
R2051 |
|
| Direct-zol™ RNA MiniPrep w/ TRI-Reagent® |
200 Preps |
R2053 |
|
| NEW Direct-zol™ RNA MicroPrep |
50 Preps |
R2060 |
|
| NEW Direct-zol™ RNA MicroPrep |
200 Preps |
R2062 |
|
| NEW Direct-zol™ RNA MicroPrep w/ TRI Reagent® |
50 Preps |
R2061 |
|
| NEW Direct-zol™ RNA MicroPrep w/ TRI Reagent® |
200 Preps |
R2063 |
|
精品推荐：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
| RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
|
|
