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Epigenetics |
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| Epigenetics>【Zymo】 |
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Direct-zol™-96 RNA（R2054） |
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Direct-zol™-96 RNA
About Direct-zol™-96 RNA
The Direct-zol™-96 RNA provides a streamlined method for the purification of up to 10 µg (per well) of high-quality RNAdirectly from samples in TRI Reagent®1. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents2.
The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin™ I96 Plate, then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization,etc.
The entire procedure typically takes about 30 minutes (per 2 plates).
*U.S. Patent No. 9,051,563 and other pending patents.
| Equipment |
Centrifuge with microplate carriers. |
| Sample Sources |
Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents. |
| Inactivation |
TRI Reagent® (provided only with R2055 & R2057) inhibits RNase activity and inactivates viruses and other infectious agents. |
| RNA Purity |
A260/A280 > 1.8, A260/A230 > 1.8. Complete removal of DNA can be performed with DNase I digestion. |
| RNA Recovery |
Typically, RNA is eluted into ≥10 µl DNase/RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg/well. |
| Compatibility |
TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
| RNA Storage |
RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage. |
| RNA Size Limits |
RNAs ≥17 nucleotides. |
Quick, 96-well purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.
| Direct-zol™-96 RNA |
2x96 preps. |
R2054 |
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| Direct-zol™-96 RNA w/ TRI Reagent® |
2x96 preps. |
R2055 |
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| Direct-zol™-96 RNA |
4x96 preps. |
R2056 |
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| Direct-zol™-96 RNA w/ TRI Reagent® |
4x96 preps. |
R2057 |
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