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Direct-zol™-96 MagBead RNA(R2100) |
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Direct-zol™-96 MagBead RNA
About Direct-zol™-96 MagBead RNA
The Direct-zol™-96 MagBead RNA provides a high-throughput, magnetic bead based purification of high-quality RNA directlyfrom samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small and non-coding RNAs (17-200 nt) is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.
The procedure is easy: simply add Direct-zol™ Binding Buffer and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
*U.S. Patent No. 9,051,563 and other pending patents.
Sample Sources |
Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents. |
RNA Purity |
A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA is performed with DNase I digestion |
RNA Recovery |
The RNA binding capacity is ~10 µg/20 µl MagBinding Beads. |
RNA Storage |
RNA eluted with the DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage. |
Equipment Needed |
Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually. |
RNA Size |
RNAs ≥17 nucleotides. |
Sample Inactivation |
TRI Reagent® (provided only with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
High-throughput, magnetic bead-based purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.)
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA
Direct-zol™-96 MagBead RNA |
2x96 Preps |
R2100 |
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Direct-zol-96 MagBead RNA w/ TRI Reagent |
2x96 Preps |
R2101 |
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Direct-zol-96 MagBead RNA |
4x96 Preps |
R2102 |
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Direct-zol-96 MagBead RNA w/ TRI Reagent |
4x96 Preps |
R2103 |
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Direct-zol-96 MagBead RNA |
8x96 Preps |
R2104 |
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Direct-zol-96 MagBead RNA w/ TRI Reagent |
8x96 Preps |
R2105 |
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