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Epigenetics |
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Epigenetics>【Zymo】 |
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Mix & Go E. coli Transformation Kit & Buffer Set---大肠杆菌高效率转化试剂盒
About Mix & Go E. coli Transformation Kit & Buffer Set
The Mix & Go E. coli Transformation Kit and Mix & Go E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go bacterial transformation can be performed by adding DNA to Mix & Go competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. colistrains.
Uniquely formulated reagents make it easy to generate Mix & Go competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go E. coli Transformation Kit includes all buffers and ZymoBroth™ medium to generate 20 ml of Mix & Go competent cells. The Mix & Go E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go competent cells, and the medium (broth) is supplied by the user.
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Make your own highly efficient chemically competent cells: 108 - 109 transformants/μg of plasmid DNA for most common lab strains.
No heat shock or related procedures: simply add DNA and spread onto a plate - Mix & Go!
Mix & Go! E.coli Transformation Kit (up to 20 ml) |
up to 20 ml |
T3001 |
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Mix & Go! E.coli Transformation Buffer Set (up to 60 ml) |
up to 60 ml |
T3002 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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