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Sox2-TAT, human recombinant |
| 中文名称 |
【Sox2】重组人Sox2-TAT蛋白（10ug） |
| 供应商 |
BioVision |
产品货号 |
7203-10 |
| 产品报价 |
客服1： 客服2： |
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购买方式
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银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系010-52406250. |
| 产品新闻 |
| 微信号：AD-Bio |
订阅号： Bio-888 |
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| 背景资料 |
Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
| 产品描述 |
该重组人Sox2-TAT蛋白产品为冻干形式.具体样式见英文说明：Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 8.0 |
| 来源宿主 |
提供的【Sox2】重组人Sox2-TAT蛋白产品的来源为大肠杆菌重组表达 . |
| 序列 |
重组人Sox2-TAT蛋白蛋白产品，又称Sox-2.其对应NCBI基因号为6657，对应Accession号为P48431.
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| 溶解建议 |
重组人Sox2-TAT蛋白产品请按照说明书建议进行溶解操作：Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| 保存建议 |
在您收到发出的【Sox2】重组人Sox2-TAT蛋白产品后，建议将其置于-20°C保存. |
| 产品说明书 |
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