PRODUCTS-Cyanagen开发的荧光染料，持续增加中ing
The names of the fluorescent dyes in the CHROMIS series are formulated using different abbreviations:
CHROMIS: Name of the class of fluorophores developed by Cyanagen.
XT: The chlorine atom in the meso position confers higher photostability to this class of fluorophores. This abbreviation lacks if the feature is not present.
Number: Three-digit number indicates the approximate absorption peak wavelength in nanometers.
Charge: A single capital letter indicates the overall charge state of the molecule (C as cationic, A as anionic, Z as zwitterionic and N as neutral).
Reactive moiety: A short description of the reactive moiety at the end of the spacer arm.
| CHROMIS |
Ex-Max (nm) |
Excitation laser line |
Commonly used filter sets |
Em-Max (nm) |
Molar absorbance (M-1cm-1) |
Quantum yield |
Quencher |
Class |
Spectrally comparable dyes* |
| 425 N |
425 |
Diode 405nm blue-violet |
DAPI |
476 |
37,000 |
Not available |
QS35 |
COUM |
PromoFluor 415 |
| 500 N |
496 |
Argon 488nm blue-green
Diode 473nm blue |
FITC |
506 |
88,000 |
1.00 |
QSY7 |
BDP |
Alexa Fluor 488
ATTO 488
BODIPY FL
Cy2
Fluorescein (FITC)
Promo Fluor 488/505 |
| 515 N |
515 |
Argon 488nm blue-green
Diode 473nm blue |
R6G |
539 |
50,000 |
0.80 |
BDP |
Alexa Fluor 514
6-JOE/6-TET
Promo Fluor 520 |
| 530 N |
529 |
Nd YAG
Diode 532nm green |
R6G |
561 |
54,000 |
0.70 |
QSY9 |
BDP |
BODIPY R6G
Alexa Fluor 532
Tetramethylrhodamine (TMR) |
| 550 C |
548 |
Nd YAG
Diode 532nm green
Helium-Neon 543nm green |
TRITC |
567 |
150,000 |
0.04 |
C5Q |
CYAN |
Alexa Fluor 546/555
BODIPY TMR
Cy3
DyLight 549
Promo Fluor 546/555
Tetramethylrhodamine (TMR) |
| 550 Z |
550 |
566 |
150,000 |
0.04 |
| 550 A |
553 |
568 |
150,000 |
0.04 |
| 555 N |
551 |
564 |
77,000 |
0.70 |
BDP |
| 570 N |
573 |
Krypton 568nm green
Helium-Neon 543nm green |
RITC |
612 |
50,000 |
0.70 |
BDP |
Alexa Fluor 568
Cy3.5
Promo Fluor 590
Texas Red |
| 580 N |
581 |
605 |
75,000 |
0.70 |
QSY21 |
ACEN |
| 590 A |
584 |
598 |
125,000 |
0.14 |
| 600 N |
593 |
Helium-Neon 594nm yellow
Diode 593.5nm yellow |
Texas Red |
624 |
50,000 |
0.60 |
BDP |
Alexa Fluor 594/610
ATTO 594
BODIPY TR
Promo Fluor 60
Texas Red |
| 630 N |
629 |
Helium-Neon 633nm red
Diode 635nm red |
|
646 |
113,000 |
0.40 |
BDP |
Alexa Fluor 633
BODIPY 630/650
Promo Fluor 633 |
645 C
645 XT C |
641 |
Krypton-Argon 647nm
Diode 635nm red |
Cy5, APC |
663 |
250,000 |
0.15÷0.25 |
CYAN |
Alexa Fluor 647
Allophycocyanin
BODIPY 650/665
Cy5
DyLight 649
Promo Fluor 647 |
645 Z
645 XT Z |
643 |
665 |
250,000 |
0.15÷0.25 |
645 A
645 XT A |
648 |
667 |
250,000 |
0.15÷0.25 |
| 670 LSS Z |
669 |
Helium-Neon laser |
CyR5.5,
LI-COR 700
channel |
778 |
90,000 |
0.10 |
|
NIR dye |
| 678 C |
679 |
Krypton 647nm red
Diode 650nm red
Diode 670nm red |
CyR5.5,
LI-COR 700
channel |
707 |
240,000 |
0.10÷0.15 |
CYAN |
Alexa Fluor 660/680
Cy5.5
Promo Fluor 680 |
| 678 Z |
678 |
705 |
195,000 |
0.10÷0.15 |
| 678 A |
677 |
703 |
240,000 |
0.10÷0.15 |
| 690 LSS Z |
668 |
Helium-Neon laser |
CyR5.5,
LI-COR 700
channel |
793 |
90,000 |
0.10÷0.15 |
|
|
NIR dye |
| 770 C |
768 |
Xenon-Arc lamp
Dye-pumped |
|
790 |
270,000 |
0.10 |
|
CYAN |
NIR dye
Promo Fluor 780 |
| 770 A |
772 |
807 |
270,000 |
0.10 |
| 800 C |
802 |
Xenon-Arc lamp
Dye-pumped |
NIR,
LI-COR 800
channel |
840 |
250,000 |
0.10÷0.15 |
|
CYAN |
NIR dye |
| 800 A |
802 |
847 |
250,000 |
0.10 |
|
| 830 C |
827 |
Xenon-Arc lamp
Dye-pumped |
|
867 |
250,000 |
0.10 |
|
CYAN |
NIR dye |
| 830 A |
826 |
851 |
200,000 |
0.10 |
|
COUM: Coumarine
BDP: Dipyrromethene borondifluoride
ACEN: Acenaphthopyrrole
CYAN: Cyanine
NOTE: This is a table of some characteristics of fluorophores useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families accepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Be sure to also look up the excitation and emission spectra for your dye of choice. |
