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Epigenetics |
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Epigenetics>【GeneMarkBio】 |
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2X PCR Dye Master Mix II(RP02D-II) |
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2X PCR Dye Master Mix II
The 2X PCR Dye Master Mix II is a ready-to-use mixture for virtually all PCR applications. The mixes contain all components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The 2X PCR Dye Master Mix II supplied as a 2X concentrated ready-to-use mix containing 0.75 u of Taq DNA polymerase, reaction buffer, 2 mM MgCl2, 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture.Store at -20°C.
Figure 1. Amplification of Enterobactria phage lambda NinB gene and xis gene using GeneMark PCR Dye Master Mix II
Gel analysis of amplified Enterobacteria phage lambda NinB gene (2 kb) and xis gene (6 kb). PCR product is directly loaded onto agarose gel.
M: GenKB DNA Ladder
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