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Epigenetics |
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Epigenetics>【GeneMarkBio】 |
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PCR Dye Plus Master Mix(RP02D-Plus) |
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PCR Dye Plus Master Mix
The PCR Dye Plus Master Mix is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixes contain GenTaq Plus™and other components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. GenTaq Plus™ is an enzyme mixture of GenTaq and Pfu DNA polymerase, it has high fidelity with an error frequency 1.6/106 during DNA synthesis and is effective over a wide range of target sizes up to 14 kb with some optimization. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Dye Plus Master Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant 1.5 u of GenTaq Plus™ polymerase, reaction buffer, MgCl2 (final concentration 2 mM), 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture. Store at -20°C.
Figure 1. Amplification of Enterobactria phage lambda ea59 gene and xis gene using GeneMark PCR Dye Plus Master Mix.
Gel analysis of amplified Enterobacteria phage lambda ea59 gene (3 kb) and xis gene (6 kb). PCR product is directly loaded onto agarose gel.
M: GenKB marker
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