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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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PCR Dye Pfu Master Mix（RP02D-pfu） |
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PCR Dye Pfu Master Mix
| The PCR Dye Pfu Master Mix is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixes contain Pfu DNA polymerase and other components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. Pfu DNA polymerase is isolated from the Pyrococus furiosus. The multifunctional thermostable enzyme possesses both of 5’ to 3’ DNA polymerase and 3’ to 5’ exonuclease activity which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Dye Pfu Master Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant 1.5 u of Pfu DNA polymerase, reaction buffer, MgSO4 (final concentration 2 mM), 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture.Store at -20°C. |
Figure 1. Amplification of Enterobactria phage lambda ea59 gene and xis gene using GeneMark’s PCR Dye Master Mix
Gel analysis of amplified Enterobacteria phage lambda ea59 gene (3 kb) and ea10 gene (1 bk). PCR product is directly loaded onto agarose gel.
M: GenKB marker
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