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Epigenetics |
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Epigenetics>【GeneMarkBio】 |
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PCR Dye Hot-Start Master Mix(RP02D-HS) |
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PCR Dye Hot-Start Master Mix
The PCR Dye Hot-Start Master Mix is a ready-to-use mixture for virtually all PCR applications. Hot-Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. The mixes contain Hot-Start Taq DNA polymerase and other components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Dye Hot-Start Master Mix II is supplied as a 5X concentrated ready-to-use mix containing 0.75 u of Hot-Start Taq DNA polymerase, reaction buffer, 2 mM MgCl2, 250 μM dNTP and enzyme in a 25 μl 1X PCR mixture. Store at -20°C.
Figure 1. Comparison of DNA amplification of Enterobactria phage lambda NinB gene using PCR Dye Master Mix II and PCR Dye Hot-Start Master Mix.
Gel electrophoresis analysis of amplified Enterobacteria phage lambda NinB gene. Non-specific bands are not observed for PCR products amplified using Hot-Start Dye Master Mix II.
M: Gen100 marker.
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