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Epigenetics |
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Epigenetics>【GeneMarkBio】 |
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One-Step RT-PCR Plus Kit (SAMscript/GenTaq Plus)(RP01-Plus) |
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One-Step RT-PCR Plus Kit (SAMscript/GenTaq Plus)
The One-Step RT-PCR Plus Kit (SAMscript RT/GenTaq Plus) is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses SAMscript reverse transcriptase for first-strand cDNA synthesis and GenTaq Plus™ for PCR amplification. SAMscript Reverse Transcriptase (SAMscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase), which lacks a 3’ to 5’ exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cDNA amplification. The kit contains GenTaq Plus™, which is an enzyme mixture of GenTaq and Pfu DNA polymerase. It has high fidelity with an error frequency of 1.6/106 during DNA synthesis, and the PCR product can be directly used for cloning to TA or expressional vectors. |
操作簡單: 只需加入DNA template及primers,減少污染機會!
快速安全: 結合 RT 反應及 PCR 反應,節省操作步驟及時間,降低人為錯誤!
方便:單一試管、單一步驟即可完成所需反應!
效率佳: 獨家反應緩衝液,可提高 RT反應溫度到50℃,克服RNA之二級結構!
敏感性高: 1-10 個 copy的RNA即可被偵測到! |
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Figure1. Gel electrophoresis analysis of RT-PCR product generated using One-step RT-PCR Plus (SAMscript/GenTaq Plus) kit.
Different concentration of RT-PCR products generated using E.coli total RNA as template is loaded and analyzed.
M: Gen100 DNA Marker
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