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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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Two-Step AMV RT-PCR Kit（RP012-A） |
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Two-Step AMV RT-PCR Kit
The GeneMark Two-Step AMV RT-PCR Kit has all the components required to perform first-strand cDNA synthesis and second strand DNA amplification. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) which catalyzes the polymerization of DNA, using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possess some RNase H activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand complementary DNA (cDNA) and primer extensions. In the cDNA synthesis step, RNA was reverse transcribed by AMV RTase to produce its cDNA. For the subsequent amplification of the cDNA template, PCR Hot-Start Master Mix II is provided. It can eliminate amplification artifacts such as primer-dimer formation and mis- priming during preamplification stage and thus may provide improved specificity. PCR Hot-Start Master Mix II can be used for effective amplification up to 6 kb DNA. The PCR Hot-Start Master Mix is supplied as a 5X concentrated ready-to-use mix that is a 1X mixture of recombinant 1.5 u of Hot Start Taq DNA polymerase, reaction buffer, 2 mM MgCl2, 250 μM dNTP and enzyme stabilizer sufficient to allow efficient amplification of template in 50 μl PCR.
產品敘述: 以RNA為模板，合成first strand cDNA，並利用Hot-start PCR mix來進行高效的2nd cDNA synthesis
酵素特性: 產品內含AMV reverse transcriptase及Hot-start PCR mix，高效率、高專一性，可於50℃下作用合成DNA (效率較M-MLV佳)
引子種類: 產品內含Oligo (dT)18及random hexamer primer供您使用 (亦可使用自行設計的專一性引子)
應用範圍: 2nd cDNA synthesis, PCR, cloning等 |
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Figure 1. RT-PCR products of E.coli total RNA generated using Two-step AMV RT-PCR Kit.
E.coli (DH5α) total RNA is used as template to generate cDNA. E.coli fruK gene is further amplified using the same kit.
M: Gen100 DNA Marker
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