Cac8 I ( BstC8 I )

Origin:
Bacillus stearothermophilus C8

Concentration:
10000, u.a./ml

Recognition site:
GCN/NGC
CGN/NCG

Reaction buffer:
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25℃); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5 -fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum: