Cla I ( BspD I, Bsa29 I )

Origin:
Bacillus stearothermophilus 29

Concentration:
20000, u.a./ml

Recognition site:
AT/CGAT
TAGC/TA

Reaction buffer:
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT + 100μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20 -fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum: