Cfr42 I

Origin:
Citrobacter freundii RFL42 

Concentration:
10 u/µl

Recognition site:
CCGC/GG
GG/CGCC

Reaction buffer:
GM-buffer I,  (10 mM Bis Tris Propane-HCl 【pH 7.0 @ 25°C】10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM KPO₄ (pH 7.4 @ 25°C), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 μg/ml BSA, 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut. 

Optimum temperature: 37°C

Inactivation 20 minutes: 65°C for 20 min.

Enzyme activity in % of maximum: