Cfr9 I

Origin:
Citrobacter freundii RFL9

Concentration:
10 u/µl

Recognition site:
C/CCGGG
GGGCC/C

Reaction buffer:
GM-buffer III,  (50 mM Tris-HCl【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 250 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, > 95% can be re-cut.

Optimum temperature: 37°C

Inactivation 20 minutes: 65°C for 20 min.

Enzyme activity in % of maximum: