EcoN I ( BstEN I )

Origin:
Bacillus stearothermophillus EM

Concentration:
2000 - 5000 u.a./ml

Recognition site:
CCTNN/NNNAGG
GGANNN/NNTCC

Reaction buffer:
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 3-fold overdigestion with enzyme more than 60% of DNA fragments can be ligated. Of these 90% can be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 65℃.

Optimum temperature:
65°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum: