EcoR I

Origin:
E. coli RY13

Concentration:
20 u/µl

Recognition site:
G/AATTC
CTTAA/G

Reaction buffer:
GM-buffer EcoR I, (10 mM Tris-HCl【pH 7.5 @ 25°C】, 50 mM NaCl, 10 mM MgCl₂, 0.025% Triton X-100)

Storage conditions:
10 mM K-PO₄ (pH 7.5 @ 25°C), 300 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.15% Triton X-100, 200 μg/ml BSA, 50% glycerol. 

Storage temperature:
-20°C

Ligation and recutting:

More than 95% of DNA fragments (1 μg) digested with 50-foldexcess enzyme can be ligated by T4 DNA ligase (400 U) at 16°Cfor 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature: 37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: