Hph I (AsuHPI)
Origin:
Haemophilus parahaemolyticus
Concentration:
5 u/µl
Recognition site:
GGTGA(N)8/
CCACT(N)7/
Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)
Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 300mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.
Storage temperature:
-20°C
Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.
Optimum temperature: 37°C
Heat inactivation: 65°C for 20 min.
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 100 |
75 |
10 |
100 |
100 |
| Product： |
| Cat. No. |
Size |
| SZ048S |
200 U |
| SZ048M |
1000 U |
  
| Product：(停產) |
| Cat. No. |
Size |
| E231 |
200 U |
| E232 |
1000 U |
|
