Nae I

Origin:
Nocardia aerocolonigenes  

Concentration:
4 u/µl

Recognition site:
GCC/GGC
CGG/CCG

Reaction buffer:
GM-buffer I, (10 mM Bis Tris propane-HCl 【pH 7.0 @ 25°C】, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl(pH 7.4 @ 25°C), 50 mM NaCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: