Nci I (AsuC2 I, Cau II)

Origin:
Actinobacillus suis CA

Concentration:
20 U/μl

Recognition site:
CC/SGG
GGS/CC

Reaction buffer:
GM-buffer II, (10 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol

Storage temperature:
-20°C

Ligation:
After AsuC2I overdigestion non of the DNA fragments can be ligated.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum: