Nhe I (AsuNH I) 

Origin:
Neisseria mucosa heidelbergensis

Concentration:
10 u/µl

Recognition site:
G/CTAGC
CGATC/G

Reaction buffer:
GM-buffer II, (10 mM Tris-HCl 【pH 7.9 @ 25°C】, 50 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl 【pH 7.4 @ 25°C】, 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, 200 μg/ml BSA and  50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: