Ple I (Pps I)
Origin:
Pseudomonas lemoignei
Concentration:
5 u/µl
Recognition site:
GAGTC(N)4/
CTCAG(N)5/
Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)
Storage conditions:
10 mM Tris-HCl (pH 7.8 @ 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol
Storage temperature:
-20°C
Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.
Optimum temperature:
37°C
Heat inactivation:
65°C for 20 min.
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 75 |
75 |
50 |
100 |
100 |
| Product |
| Cat. No. |
Size |
| SZ068S |
200 U |
| SZ068M |
1000 U |
| SZ068L |
5000 U |
|
