Pst I

Origin:
Providencia stuartii 164

Concentration:
20 u/µl

Recognition site:
CTGCA/G
G/ACGTC

Reaction buffer:
GM-buffer III,  (50 mM Tris-HCl 【pH 7.9 @ 25°C】, 100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 200 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA, 50% glycerol

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.  

Optimum temperature:
37°C

Heat inactivation:
80°C for 20 min.

Enzyme activity in % of maximum: