Rsa I (Afa I)

Origin:
Rhodopseudomonas sphaeroides

Concentration:
10 u/µl

Recognition site:
GT/AC
CA/TG

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: