Sac I (Psp124BI, Sst I)

Origin:
Streptomyces achromogenes

Concentration:
20 u/µl

Recognition site:
GAGCT/C
C/TCGAG

Reaction buffer:
GM-buffer I, (10 mM Bis Tris propane-HCl 【pH 7.0 @ 25°C】, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation:
65°C for 20 min.

Enzyme activity in % of maximum: