Sac II (Sfr303I, SgrBI, SlaI)
Origin:
Streptomyces fradiae 303
Concentration:
5000 - 20000, u.a./ml
Recognition site:
CCGC/GG
GG/CGCC
Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
| B |
G |
O |
W |
Y |
| 100 |
50-75 |
10-25 |
10-25 |
75-100 |
| Product |
| Cat. No. |
Size |
| SZS100S |
400 U |
| SZS100M |
1000 U |
| SZS100L |
5000 U |
|
