Sal I
Origin:
Streptomyces albus G
Concentration:
20 u/µl
Recognition site:
G/TCGAC
CAGCT/G
Reaction buffer:
GM-buffer III, (50 mM Tris-HCl 【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA)
Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 300 μg/ml BSA, 50% glycerol.
Storage temperature:
-20°C
Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.
Optimum temperature:
37°C
Heat inactivation:
65°C for 20 min.
Enzyme activity in % of maximum:
I |
II |
III |
IV |
ONE |
0 |
0 |
100 |
0 |
100 |
Product |
Cat. No. |
Size |
SZ074S |
1000 U |
SZ074M |
5000 U |
SZ074L |
25000 U |
|