Sap I (PciS I)

Origin:
Planococcus citreus S

Concentration:
500 - 2000, u.a./ml

Recognition site:
GCTCTTC(N)₁/
CGAGAAG(N)₄/

Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 3-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated with T4 DNA Ligase at 16°C and of these 95% can be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 1 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum: