Sfi I

Origin:
Streptomyces fimbriatus

Concentration:
4 u/µl

Recognition site:
GGCCNNNN/NGGCC
CCGGN/NNNNCCGG

Reaction buffer:
GM-buffer II,  (10 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
20 mM Tris-HCl (pH 7.4 @ 25°C), 300 mM NaCl, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, 0.15% Triton X-100, 200 μg/ml BSA, 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: No.

Enzyme activity in % of maximum: