Sse9 I

Origin:
Sporosarcina species

Concentration:
5 u/µl

Recognition site:
N/AATTN

Reaction buffer:
GM-buffer I,  (10 mM Bis Tris propane-HCl 【pH 7.0 @ 25°C】,  10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation:
65°C for 20 min.

Enzyme activity in % of maximum: